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It was this moment that Peter Parker learned his lesson. He acted irresponsible, and he lost Uncle Ben:
A legend is born with the death of Uncle Ben, he learns that, “In this world, with great power there must also come -- great responsibility.”
Spider-Man's responsibility (to do the right thing) is based on guilt.
I not once used the word grief. I said guilt.
People riddled with guilt will spend the rest of their lives doing good deeds trying to make up for it (as atonement for their sins).
You are right that love is part of the mix, but guilt is the primary ingredient.