Garrett Hedlund as Leonardo (voice)
Ian Somerhader as Raphael (voice)
Andrew Garfield as Donatello (voice)
Matt Smith as Michelangelo (voice)
Cary Hiroyuki Tagawa as sensei Splinter (voice)
Lauren Lee Smith as April O'Neil
Bryce Dallas Howard as April O'Neil
Brit Marling as April O'Neil
Jennifer Morrison as April O'Neil
Mary Elizabeth Winstead as April O'Neil
Sienna Guillory as April O'Neil
Claire Danes as April O'Neil
Amanda Righetti as April O'Neil
Amy Adams as April O'Neil
Taylor Kitsch as Casey Jones
Ken Watanabe as Shredder/Oroku Saki
Maggie Q as Karai
Chiwetel Ejiofor as Dr. Baxter Stockman
Frankie Muniz as Cody Jones O'Neil